DB53/T 1059.3-2021 滇金丝猴检疫技术 第3部分：隐孢子虫实验室检测技术规范
DB53/T 1059.3-2021 DB53/T 1059.3-2021 "Yunnan golden snub-nosed monkey quarantine technology Part 3: Laboratory detection technical specifications for cryptosporidium"
基本信息
发布历史
-
2021年09月
研制信息
- 起草单位：
- 起草人：
- 出版信息：
- 页数：18页 | 字数：- | 开本： -
内容描述
ICS11.220
ICSB41
53
云南省地方标准
DB53/T1059.3—2021
滇金丝猴检疫技术
第3部分：隐孢子虫实验室检测技术规范
QuarantinetechniqueforRhinopithecusbieti
—Part3:TechnicalspecificationsforlaboratoryexaminationofCryptosporidium
2021-09-30发布2021-10-14实施
云南省市场监督管理局发布
DB53/T1059.3—2021
目 次
前言...................................................................................................................................................................III
引言.....................................................................................................................................................................Ⅴ
1范围.................................................................................................................................................................1
2规范性引用文件.............................................................................................................................................1
3术语和定义.....................................................................................................................................................1
4缩略语.............................................................................................................................................................2
5试验原理.........................................................................................................................................................2
6试验条件.........................................................................................................................................................2
6.1实验室通用要求.....................................................................................................................................2
6.2采样人员要求.........................................................................................................................................2
7仪器设备和试剂.............................................................................................................................................2
7.1仪器设备.................................................................................................................................................2
7.2试剂.........................................................................................................................................................2
8样品.................................................................................................................................................................3
8.1采样要求.................................................................................................................................................3
8.2粪样的选取原则.....................................................................................................................................3
8.3采样程序.................................................................................................................................................3
8.4样品的保存与运输.................................................................................................................................3
9试验步骤.........................................................................................................................................................3
9.1形态学检测.............................................................................................................................................4
9.2巢式PCR检测.........................................................................................................................................4
10试验数据处理...............................................................................................................................................5
附录A（规范性）粪样的采集信息表............................................................................................................6
附录B（规范性）巢式PCR反应....................................................................................................................7
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