T/LNSES 001-2023 场地土壤环境损害调查技术指南 ——土壤线虫调查和评价

ICS13.080

CCSZ00

团体标准

T/LNSES001-2023

场地土壤环境损害调查技术指南

——土壤线虫调查和评价

TechnicalGuidelinesforInvestigationofSiteSoilEnvironmentalDamage:Soil

NematodesInvestigationandEvaluation

2023-02-01发布2023-02-01实施

辽宁省环境科学学会  发布

T/LNSES001-2023

目次

前言......................................................................................................................................................................II

1适用范围..............................................................................................................................................................1

2规范性引用文件..................................................................................................................................................1

3术语和定义..........................................................................................................................................................1

4基本原则..............................................................................................................................................................1

4.1科学性原则..............................................................................................................................................1

4.2可操作性原则..........................................................................................................................................1

4.3持续性原则..............................................................................................................................................1

4.4保护性原则..............................................................................................................................................1

4.5安全性原则..............................................................................................................................................2

5材料试剂和仪器设备..........................................................................................................................................2

5.1材料试剂..................................................................................................................................................2

5.2仪器设备..................................................................................................................................................2

6土壤线虫调查方法..............................................................................................................................................2

6.1调查程序..................................................................................................................................................2

6.2样地和样方设置......................................................................................................................................3

6.3土壤线虫取样方法..................................................................................................................................3

6.4土壤线虫分离提取方法..........................................................................................................................4

6.5土壤线虫标本制作方法..........................................................................................................................5

6.6土壤线虫计数和科属鉴定......................................................................................................................5

7土壤线虫群落评价方法......................................................................................................................................6

7.1土壤线虫群落多样性指数......................................................................................................................6

7.2土壤线虫功能多样性指数......................................................................................................................7

8土壤线虫结构与功能完整性评价方法.............................................................................................................7

9土壤线虫数据处理和分析..................................................................................................................................9

10质量控制............................................................................................................................................................9

11报告编制............................................................................................................................................................9

附录A（规范性）调查场地生境要素记录表..................................................................................................10

附录B（规范性）样地、样方记录表...............................................................................................................11

附录C（规范性）土壤线虫分类检索文献.......................................................................................................12

附录D（资料性）土壤线虫群落评价指数的原理和优势..............................................................................13

I

T/LNSES001-2023

前言

本文件按照GB/T1.1-2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定起

草。

本文件由辽宁省环境科学学会提出并归口。

本文件主要起草单位：沈阳建筑大学、东北师范大学。

本文件主要起草人：郑晓雪、王忠强、唐玉兰、施瑶、李婷、黄殿男、傅金祥、由昆、刘强、杨

辉、霍忠奇、李微、魏婕、于靓、张萧汉、葛成林、王坤。

本文件附录A、附录B和附录C为规范性附录，附录D为资料性附录。

II

T/LNSES001-2023