GB/T 45033-2024 饲用微生物制剂中产朊假丝酵母的测定

ICS

65.120

CCS

B46

中华人民共和国国家标准

GB/T45033—2024

饲用微生物制剂中产朊假丝酵母的测定

DeterminationofCandidautilisinmicrobialfeedadditives

2024-11-28发布2025-06-01实施

国家市场监督管理总局发布

国家标准化管理委员会

GB/T45033—2024

目次

前言

·····································································································

Ⅲ

1

范围

··································································································

1

2

规范性引用文件

······················································································

1

3

术语和定义

···························································································

1

4

试剂或材料

···························································································

1

5

仪器和设备

···························································································

1

6

采样

··································································································

1

7

试验步骤

······························································································

2

8

试验数据处理

·························································································

3

附录A（资料性）

培养基

·············································································

5

附录B（规范性）

生理生化试验

······································································

6

附录C（规范性）

分子生物学鉴定

···································································

9

Ⅰ

GB/T45033—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由全国饲料工业标准化技术委员会（SAC/TC76）提出并归口。

本文件起草单位：山东宝来利来生物工程股份有限公司、安琪酵母股份有限公司、北京大北农科技

集团股份有限公司、浙江科峰生物技术有限公司、唐山拓普生物科技有限公司、益海嘉里（防城港）生

物科技有限公司。

本文件主要起草人：谷巍、单宝龙、徐海燕、谢全喜、辛国芹、汪祥燕、亓秀晔、覃先武、刘雪连、

章亭洲、周雪玲、马立周。

Ⅲ

GB/T45033—2024

饲用微生物制剂中产朊假丝酵母的测定

1范围

本文件描述了饲用微生物制剂中产朊假丝酵母（Candidautilis）菌数的测定方法。

本文件适用于含有产朊假丝酵母饲用微生物制剂的测定。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用

于本文件。

GB/T6682分析实验室用水规格和试验方法

GB/T42959饲料微生物检验采样

3术语和定义

本文件没有需要界定的术语和定义。

4试剂或材料

除非另有规定，仅使用分析纯试剂。

4.1水：GB/T6682，三级。

4.20.85%灭菌生理盐水：准确称取8.5g氯化钠加入1000mL水中，搅拌至完全溶解，分装后，121℃

灭菌30min，备用。

4.3孟加拉红琼脂培养基：见附录A中A.1。

4.4酵母膏胨葡萄糖（YPD）琼脂培养基：见A.2。

5仪器和设备

除微生物实验室常规灭菌及培养设备外，其他仪器设备如下。

5.1培养箱：可控精度±1℃。

5.2天平：感量为0.1g。

5.3光学显微镜：400倍～1000倍。

5.4拍击式均质器及无菌均质袋。

5.5旋涡混合器。

5.6无菌培养皿：直径90mm。

5.7聚合酶链式反应（PCR）仪。

6采样

按照GB/T42959执行。

1