DB42/T 1753-2021 滇楸 组培苗生产技术规程

ICS65.020.40

CCSB61

DB42

湖北省地方标准

DB42/T1753—2021

滇楸组培苗生产技术规程

Codeofpracticefortheproductionoftissuecultureseedlingsof

CatalpafargesiiBur.f.duclouxii(Dode)Gilmour

2021-08-30发布2021-10-30实施

湖北省市场监督管理局发布

DB42/T1753—2021

目次

前言.................................................................................II

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4繁殖材料要求.......................................................................1

5外植体采集与处理...................................................................1

采集...........................................................................1

处理...........................................................................1

6培养前准备.........................................................................2

超净台消毒.....................................................................2

培养基配制与灭菌...............................................................2

培养基保存.....................................................................2

7组织培养...........................................................................2

培养条件.......................................................................2

初代培养.......................................................................2

继代培养.......................................................................2

生根培养.......................................................................3

外植体更新.....................................................................3

8室内炼苗...........................................................................3

组培苗要求.....................................................................3

炼苗方法.......................................................................3

9移栽...............................................................................3

移栽基质.......................................................................3

组培苗清洗.....................................................................3

废弃培养基处理.................................................................3

植入容器.......................................................................3

容器苗管理.....................................................................3

10技术档案管理......................................................................4

附录A（资料性）滇楸组培苗培养基配方表.