DB42/T 1448-2018 蝴蝶兰组培苗工厂化生产技术规程

ICS65.020

B62

备案号：DB42

湖北省地方标准

DB42/T1448—2018

蝴蝶兰组培苗工厂化生产技术规程

Codeofpracticefortissuecultureofphalaenopsisseedlinginindustrialproduction

（报批稿）

2018-09-11发布2018-12-10实施

湖北省质量技术监督局发布

1

DB42/T1448—2018

目次

前言................................................................................III

1范围................................................................................................................................................................1

2规范性引用文件............................................................................................................................................1

3术语与定义....................................................................................................................................................1

3.1组培苗invitroplantlet........................................................................................................................1

3.2中苗mediumsizedplantlet..................................................................................................................1

3.3成品苗thefinishedproductplantlet....................................................................................................1

4设施条件........................................................................................................................................................1

5培养基配制....................................................................................................................................................2

5.1水质要求.................................................................................................................................................2

5.2培养基配制及分装.................................................................................................................................2

5.3培养基灭菌.............................................................................................................................................2

5.4培养基储存.............................................................................................................................................2

6组织培养操作方法........................................................................................................................................2

6.1操作前准备.............................................................................................................................................2

6.2外植体选择与消毒.................................................................................................................................2

6.3增殖与继代培养.....................................................................................................................................3

6.4壮苗培养.................................................................................................................................................3

6.5生根培养.................................................................................................................................................3

6.6接种.........................................................................................................................................................4

7组培苗培养管理............................................................................................................................................4

7.1组培苗摆放....................................................................