GB/T 26347-2026 蚊虫抗药性检测方法 生物测定法

ICS11.020

CCSC05

中华人民共和国国家标准

GB/T26347—2026

代替GB/T26347—2010

蚊虫抗药性检测方法生物测定法

Testmethodofmosquitoresistancetoinsecticides—Bioassaymethod

2026⁃07⁃02发布2027⁃02⁃01实施

国家市场监督管理总局

国家标准化管理委员会发布

GB/T26347—2026

目次

前言··························································································································Ⅲ

1范围·······················································································································1

2规范性引用文件········································································································1

3术语和定义··············································································································1

4试虫·······················································································································1

4.1敏感品系···········································································································1

4.2待测种群···········································································································2

5试剂·······················································································································2

6器材·······················································································································2

7测试条件·················································································································2

8蚊虫抗药性水平测定方法····························································································2

8.1敏感基线法········································································································2

8.2诊断剂量法········································································································4

9基于增效剂PBO与杀虫剂联用的抗药性类型测定方法······················································5

9.1操作步骤···········································································································5

9.2死亡判断···········································································································5

9.3结果表述···········································································································5

9.4结果判定···········································································································5

附录A（资料性）世界卫生组织（WHO）推荐使用的接触筒····················································7

附录B（资料性）世界卫生组织（WHO）推荐使用的玻璃瓶····················································8

附录C（资料性）主要杀虫剂对蚊虫的诊断剂量··································································9

附录D（资料性）基于增效剂与杀虫剂联用的抗药性类型测定示意图·····································11

Ⅰ

GB/T26347—2026

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

本文件代替GB/T26347—2010《蚊虫抗药性检测方法生物测定法》，与GB/T26347—2010相

比，除结构调整和编辑性改动外，主要技术变化如下：

a）删除了“敏感基线”“F1代”2个术语（见2010年版的2.1、2.3）；

b）增加了“敏感品系”“待测种群”“诊断时间”“增效剂”4个术语（见3.1、3.2、3.4、3.5）；

c）更改了术语“诊断剂量”（见3.3，2010年版的2.2）；

d）更改了试剂和器材的要求（见第5章和第6章，2010年版的第4章和第5章）；

e）增加了数据处理和死亡判断的表述（见8.1.4和8.2.3）；

f）增加了玻璃瓶法的测定方法（见8.2.2.2.2）；

g）更改了诊断剂量法的结果判定（见8.2.4，2010年版的8.4）；

h）增加了基于增效剂PBO与杀虫剂联用的抗药性类型测定（见第9章）。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由中华人民共和国国家卫生健康委员会提出并归口。

本文件起草单位：中国人民解放军军事科学院军事医学研究院、北京市疾病预防控制中心、中国疾

病预防控制中心传染病预防控制所、中国农业大学、大连国际旅行卫生保健中心、南京市疾病预防控制

中心。

本文件主要起草人：赵彤言、李春晓、董言德、曾晓芃、孟凤霞、高希武、宋锋林、佟颖、刘美德、

张守刚。

本文件及其所代替文件的历次版本发布情况为：

——2011年首次发布为GB/T26347—2010；

——本次为第一次修订。

Ⅲ

GB/T26347—2026

蚊虫抗药性检测方法生物测定法

1范围

本文件规定了蚊虫对有机磷类、氨基甲酸酯类、拟除虫菊酯类等杀虫剂抗药性检测时，试虫、试剂、

器材、测试条件的要求，描述了蚊虫抗药性水平的测定方法和基于增效剂PBO与杀虫剂联用的抗药性

类型测定方法。

本文件适用于淡色库蚊（Culexpipienspallens）、致倦库蚊（Cx.pipiensquinquefasciatus）、白纹伊蚊

（Aedesalbopictus）、埃及伊蚊（Aedesaegypti）、中华按蚊（Anophelessinensis）等蚊虫抗药性的生物测定。

2规范性引用文件

本文件没有规范性引用文件。

3术语和定义

下列术语和定义适用于本文件。

3.1

敏感品系susceptiblestrain

由于未曾接触过杀虫剂而保持对杀虫剂具有敏感状态，遗传上较均一的试虫种群。

3.2

待测种群testpopulation

从野外（现场）特定地点和时间采集的、需要进行杀虫剂敏感性测试的目标昆虫群体。

3.3

诊断剂量diagnosticdose

99%99.9%[99%LD99.9%

能杀死媒介生物敏感品系或个体的杀虫剂剂量致死量（99）或致死量

LD][99%LC99.9%LC]

（99.9）或浓度值致死浓度（99）或致死浓度（99.9）的数倍。

注：用以区分敏感和抗性种群。

3.4

诊断时间diagnostictime

成蚊玻璃瓶法中某剂量杀虫剂杀死全部敏感品系蚊虫的时间。

3.5

增效剂synergist

本身无生物活性，但能与杀虫剂混用显著提升其毒力与药效的助剂。

4试虫

4.1敏感品系

实验室标准化饲养的形态完整、活动灵敏、体型大小一致的蚊虫。

1