GB/T 44707-2024 化学品 强化快速生物降解性试验

ICS

13.300

CCS

A80

中华人民共和国国家标准

GB/T44707—2024

化学品强化快速生物降解性试验

Chemicals—Enhancedreadybiodegradabilitytest

2024-10-26发布2025-05-01实施

国家市场监督管理总局发布

国家标准化管理委员会

GB/T44707—2024

目次

前言

·····································································································

Ⅲ

引言

·····································································································

Ⅳ

1

范围

··································································································

1

2

规范性引用文件

······················································································

1

3

术语和定义

···························································································

1

4

方法概述

······························································································

2

4.1

原理

······························································································

2

4.2

方法选择

·························································································

2

5

受试物信息

···························································································

2

6

参比物

································································································

3

7

试验准备

······························································································

3

7.1

仪器设备

·························································································

3

7.2

试验用水和培养基

···············································································

3

7.3

接种物

···························································································

3

8

试验程序

······························································································

4

8.1

强化条件

·························································································

4

8.2

受试物的添加

····················································································

4

8.3

试验操作

·························································································

4

9

质量保证与质量控制

·················································································

4

10

数据

·································································································

5

11

结果报告

····························································································

5

参考文献

··································································································

6

Ⅰ

GB/T44707—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

本文件与GB/T27850《化学品快速生物降解性通则》、GB/T21801《化学品快速生物降解

性呼吸计量法试验》、GB/T21802《化学品快速生物降解性改进的MITI试验（Ⅰ）》、GB/T21803

《化学品快速生物降解性DOC消减试验》、GB/T21831《化学品快速生物降解性：密闭瓶法试

验》、GB/T21856《化学品快速生物降解性二氧化碳产生试验》和GB/T21857《化学品快速

生物降解性改进的OECD筛选试验》配套使用。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由全国危险化学品管理标准化技术委员会（SAC/TC251）提出并归口。

本文件起草单位：广东省科学院微生物研究所（广东省微生物分析检测中心）、生态环境部固体废

物与化学品管理技术中心、中检科健（天津）检验检测有限责任公司、路博润管理（上海）有限公司、

上海市检测中心、生态环境部南京环境科学研究所、上海化工院检测有限公司、沈阳沈化院测试技术有

限公司、中国合格评定国家认可中心、万华化学集团股份有限公司、福斯润滑油（中国）有限公司、能

特科技有限公司。

本文件主要起草人：许玫英、梅承芳、吴孝槐、邓桂荣、刘纯新、曾国驱、罗凤娟、周丽丽、

滕晓明、邢维龙、舒耀皋、戎志毅、叶芷君、杨雪菲、周彬彬、孙帅、乔建成。

Ⅲ

GB/T44707—2024

引言

当化合物在快速生物降解性试验中表现为不具有快速生物降解性时，需要进一步开展更多的筛选性

降解试验，如强化快速生物降解性试验、固有生物降解性试验等，以识别是否具有潜在的持久性。推荐

采用强化快速生物降解性试验评估持久性，其方法选择更广、优化条件更全面。

本文件与快速生物降解性通则（GB/T27850）及任一快速生物降解性试验方法（GB/T21801、

GB/T21802、GB/T21803、GB/T21831、GB/T21856、GB/T21857和OECD化学品测试导则

No.310）联合使用，将所选用的快速生物降解性试验标准作为基础方法，通过对一个或多个试验条件

进行强化开展测试。除本文件描述的内容之外，其他试验内容和要求见所选用的方法标准。

如采用本文件通过预暴露接种物或增加接种物浓度开展测试并将获得的数据用于监管时，需考虑试

验结果是否充分且有效。

Ⅳ

GB/T44707—2024

化学品强化快速生物降解性试验

1范围

本文件描述了化学品强化快速生物降解性试验的方法概述、受试物信息、参比物、试验准备、试验

程序、质量保证与质量控制、数据、结果报告。

本文件适用于化学品的强化快速生物降解性试验。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用

于本文件。

GB/T21801化学品快速生物降解性呼吸计量法试验

GB/T21802化学品快速生物降解性改进的MITI试验（Ⅰ）

GB/T21803化学品快速生物降解性DOC消减试验

GB/T21831化学品快速生物降解性：密闭瓶法试验

GB/T21856化学品快速生物降解性二氧化碳产生试验

GB/T21857化学品快速生物降解性改进的OECD筛选试验

GB/T27850化学品快速生物降解性通则

OECD化学品测试导则No.310

快速生物降解性﹘密闭瓶二氧化碳法（顶空试验）[ReadyBiodegrad﹘

ability﹘CO2insealedvessels(HeadspaceTest)]

3术语和定义

下列术语和定义适用于本文件。

3.1

生物降解性biodegradability

受试化合物与接种物接触后表现出的生物降解能力。

3.2

快速生物降解性readybiodegradability

受试化合物在限定时间内与接种物接触后表现出的生物降解能力。

3.3

持久性persistence

受试化合物在环境中保持不变的能力或在环境中不分解不转化的趋势。

注：通常依据受试物在环境介质中的半衰期和/或生物降解率进行评估。

3.4

预调节pre﹘conditioning

受试化合物不存在时，在试验条件下对接种物进行曝气培养。

注：通过减少接种物空白值和增强微生物对试验条件的适应性来提高试验质量。

3.5

预暴露pre﹘exposure

受试化合物存在时，在试验条件下对接种物进行预培养。

1