DB35/T 1218-2011 马铃薯晚疫病菌生理小种鉴定技术规程

ICS65.020

B16DB35

福建省地方标准

DB35/T1218—2011

马铃薯晚疫病菌生理小种鉴定技术规程

Technicalstandardforidentificationonthephysiologicalracesofphytophthora

infestansonpotato

2011-12-31发布2012-03-15实施

福建省质量技术监督局发布

DB35/T1218—2011

目次

前言................................................................................II

1范围..............................................................................1

2术语和定义........................................................................1

3接种体的制备......................................................................1

3.1培养基的配制..................................................................2

3.1.1黑麦培养基................................................................2

3.1.2选择性培养基..............................................................2

3.1.3MS培养基..................................................................2

3.2晚疫病样的采集................................................................2

3.3晚疫病菌的分离................................................................2

3.3.1保湿培养分离法............................................................2

3.3.2薯片夹叶分离法............................................................2

3.4晚疫病菌的鉴定与纯化..........................................................2

3.5晚疫病菌的保存................................................................2

3.6孢子囊悬浮液的制备............................................................2

4鉴别寄主的保存与扩繁..............................................................3

4.1保存..........................................................................3

4.2扩繁..........................................................................3

5接种鉴定..........................................................................3

5.1接种方法......................................................................3

5.2接种技术......................................................................3

5.3接种后的培养..................................................................3

6病情调查..........................................................................3

6.1调查时间......................................................................3

6.2病情调查方法..................................................................3

7生理小种确定.