GB/T 44349-2024 蜂花粉总多酚的检测 福林酚试剂 比色法

ICS

65.140

CCS

B47

中华人民共和国国家标准

GB/T44349—2024

蜂花粉总多酚的检测福林酚试剂

比色法

Detectionoftotalpolyphenolsinbeepollen—Colorimetricmethodusing

Folin-Ciocalteureagent

2024-09-29发布2025-04-01实施

国家市场监督管理总局发布

国家标准化管理委员会

GB/T44349—2024

目次

前言

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1

范围

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2

规范性引用文件

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3

术语和定义

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4

原理

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5

试剂和材料

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6

仪器设备

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7

分析步骤

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7.1

试样制备

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7.2

试样前处理

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7.3

空白试验

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7.4

标准曲线的制作

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7.5

试样测定

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结果计算

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9

定量限

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精密度

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附录A（资料性）

福林酚试剂（Folin﹘Ciocalteureagent）配制方法

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参考文献

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Ⅰ

GB/T44349—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由中华全国供销合作总社提出。

本文件由全国蜂产品标准化技术委员会（SAC/TC601）归口。

本文件起草单位：中国计量大学、安徽省王巢食品有限公司、广东正当年生物科技股份有限公司、

中科标准（宁德）科技有限公司、睿科集团（厦门）股份有限公司、深圳职业技术大学、秦皇岛海关技

术中心、阜阳职业技术学院、南京众谱生物科技有限公司、浙江省农业科学院、浙江省中医药研究院、

中国农业科学院蜜蜂研究所、青海省花宝蜂业股份合作公司、杭州天厨蜜源保健品有限公司、兰溪市产

品商品质量检测研究院、山东世通检测评价技术服务有限公司、陕西国蜂大健康产业有限公司、江西汪

氏蜜蜂园有限公司。

本文件主要起草人：李红亮、胡童霞、岳振峰、吴帆、崔宗岩、徐锦忠、曹联飞、韩亚超、

张敬群、王磊、赵光培、蒋先志、陈璇、李强强、董捷、李平、林影、李斌、车延年、陈云、汪玲。

Ⅲ

GB/T44349—2024

蜂花粉总多酚的检测福林酚试剂

比色法

1范围

本文件描述了测定蜂花粉中总多酚的福林酚试剂比色方法。

本文件适用于蜂花粉中总多酚含量的测定。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用

于本文件。

GB5009.3—2016食品安全国家标准食品中水分的测定

GB/T6682分析实验室用水规格和试验方法。

3术语和定义

下列术语和定义适用于本文件。

3.1

蜂花粉beepollen

工蜂采集花粉，用唾液和花蜜混合后形成的物质。

[来源：GB/T30359—2021，3.2]

4原理

蜂花粉经75%乙醇溶液超声提取的总多酚物质的酚羟基在碱性条件下，与福林酚试剂中磷钨钼酸的

六价钨离子（W6+）反应生成蓝色的五价钨离子（W5+），可在765nm波长处产生最大吸收。在一定浓

度范围内，其吸光度与总多酚类化合物含量成正比。与没食子酸标准曲线比较，可定量测定总多酚化合

物的含量。

5试剂和材料

除非另有规定，仅使用分析纯试剂。

5.1水，GB/T6682，三级。

5.2无水乙醇。

5.3无水碳酸钠。

5.4福林酚试剂（Folin﹘Ciocalteureagent）：1mol/L（或1N），以棕色瓶分装，4℃避光保存。直

接购买或配制，配制方法见附录A。

5.575%（体积分数）乙醇溶液：加25mL水至75mL无水乙醇（5.2）中，混合摇匀。

5.6碳酸钠溶液（10.0%）：称取10.00g±0.02g无水碳酸钠（5.3），加水溶解并稀释至100mL，

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