DB46/T 644-2024 咖啡果小蠹快速分子鉴定技术规程

ICS65.020．99

CCSB16

46

海南省地方标准

DB46/T644—2024

咖啡果小蠹分子鉴定技术规程

Technicalcodeformolecularidentificationofcoffeeberryborer

2024-09-23发布2024-11-01实施

海南省市场监督管理局发布

DB46/T644—2024

目次

前言................................................................................III

引言.................................................................................IV

1范围................................................................................1

2规范性引用文件......................................................................1

3术语与定义..........................................................................1

4缩略语..............................................................................1

5咖啡果小蠹基本信息..................................................................1

6原理................................................................................1

7主要仪器设备和主要试剂..............................................................1

主要仪器设备....................................................................1

试剂............................................................................2

8分子鉴定操作步骤....................................................................2

样品处理........................................................................2

DNA提取.........................................................................2

DNA纯度和浓度测定...............................................................2

特异性引物使用..................................................................2

PCR扩增反应.....................................................................2

电泳及测序......................................................................2

9结果判定............................................................................2

10样品保存、复核与记录...............................................................2

样品保存.......................................................................2

复核...........................................................................2

记录...........................................................................2

附录A（资料性）咖啡果小蠹基本信息...................................................3

A.1生物学特性......................................................................3

A.2传播途径........................................................................3

A.3地理分布........................................................................3

A.4寄主............................................................................4

附录B（规范性）DNA提取方法及PCR检测方法...........................................5

B.1DNA提取方法.....................................................................5

B.2引物序列........................................................................5

B.3扩增反应体系....................................................................5

B.4扩增反应条件....................................................................5

I

DB46/T644—2024

B.5PCR扩增片段电泳结果.............................................................5

B.6PCR扩增片段测序结果.............................................................6

B.7结果判定.........................................................................6

II

DB46/T644—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的

规定起草。

请注意本文件的某些内容可能