DB43/T 2193-2021 水环境中吗咖和 O6-单乙吗咖含量的测定 液相色谱-串联质谱法

ICS19.020

CCSN50

43

湖南省地方标准

DB43/T2193—2021

水环境中吗啡和O-6单乙酰吗啡含量的测定

液相色谱-串联质谱法

DeterminationofmorphineandO6-acetylmorphineinwaterenvironment

withliquidchromatography-tandemmassspectrometry

2021-11-09发布2022-01-09实施

湖南省市场监督管理局发布

DB43/T2193—2021

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3定义·····················································································································1

4方法原理···············································································································1

5试剂和材料············································································································1

6仪器和设备············································································································2

7样品制备和保存······································································································3

8分析步骤···············································································································3

9结果计算与表示······································································································6

10方法检出限和定量限······························································································6

附录A（资料性）吗啡(Morphine）和O6-单乙酰吗啡(O6-Acetylmorphine）及其氘代内标的MRM色谱图

································································································································7

I

DB43/T2193—2021

II

DB43/T2193—2021

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由湖南省市场监督管理局提出并归口。

本文件起草单位：湖南省产商品质量检验研究院和湖南省毒品检验分析室（长沙市公安局毒品检验

鉴定中心）。

本文件主要起草人：刘红梅、张旭东、覃汉兵、李欣、肖蓉、钱荣敬、张蕾、唐超群、陈实、叶小

兰、李志丰。

III

DB43/T2193—2021

IV

DB43/T2193—2021

6

水环境中吗啡和O-单乙酰吗啡含量的测定液相色谱-串联质谱法

1范围

本文件规定了水环境样品中吗啡和O6-单乙酰吗啡的测定方法。

本文件适用于水环境样品中吗啡和O6-单乙酰吗啡含量的测定。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

GB/T603化学试剂试验方法中所用制剂及制品的制备

GB/T6682分析实验室用水规格和试验方法

HJ/T91.1污水监测技术规范

DB43/T2199水环境中12种毒品及其代谢物、人口标记物检验方法总则

3定义

3.1

水环境waterenvironment

水环境是指自然界中水的形成、分布和转化所处空间的环境，是指围绕人群空间及可直接或间接影

响人类生活和发展的水体，其正常功能的各种自然因素和有关的社会因素的总体。本文件中的水环境指

的是生活污水、工业废水和自然水体（地表水和地下水）。

4方法原理

向样品中添加同位素内标，样品中的毒品及其代谢物经离线或在线固相萃取柱富集，采用液相色谱

-串联质谱法进行检测，以保留时间（tR）和质谱特征碎片离子进行定性分析，同位素内标法进行定量

分析。

5试剂和材料

5.1试剂

5.1.1通用要求

实验用水符合GB/T6682相关规范，除非另有说明，所用试剂均为分析纯试剂。本标准中所用试剂

和溶液的配制，在未注明规格和配制方法时，均应按GB/T603之规定执行。

5.1.2吗啡和O6-单乙酰吗啡标准溶液（ρ=1mg/mL）：可用标准物质配制或直接购买有证标准溶液，

1

DB43/T2193—2021

使用标准物质配制时应根据标准物质的纯度和盐型进行换算，于-20℃避光、密封保存，有效期为1

年。

5.1.3吗啡和O6-单乙酰吗啡混合标准储备溶液（ρ=10mg/L）：将吗啡和O6-单乙酰吗啡的标准溶液用

甲醇稀释，配制成浓度为10mg/L的混合标准储备溶液。于-20℃避光、密封保存，有效期为6个月，

使用时应恢复至室温，并摇匀。

5.1.4吗啡和O6-单乙酰吗啡氘代内标标准溶液（ρ=0.1mg/mL）：可用标准物质配制或直接购买有证

标准溶液，使用标准物质配制时应根据标准物质的纯度和盐型进行换算，于-20℃避光、密封保存，有

效期为1年。

5.1.5吗啡和O6-单乙酰吗啡混合氘代内标储备溶液（ρ=1mg/L）：将吗啡和O