DB62/T 2531-2014 陇南市天麻萌发菌、蜜环菌培养技术规程

ICS65.020.20

B05

DB62

甘肃省地方标准

DB62/T2531—2014

陇南市天麻萌发菌、蜜环菌培养技术规程

2014-12-23发布2015-01-20实施

发布

DB62/T2531—2014

目次

前言...............................................................................III

1范围..............................................................................1

2规范性引用文件....................................................................1

3术语和定义........................................................................2

4萌发菌、蜜环菌的培养..............................................................2

4.1培养条件......................................................................2

4.2菌种培养......................................................................2

4.2.1萌发菌、蜜环菌一级菌种（母种）培养........................................2

4.2.1.1PDA培养基制备.........................................................2

4.2.1.2菌种选择..............................................................2

4.2.1.3分离方法..............................................................2

4.2.1.4菌种纯化..............................................................3

4.2.2萌发菌、蜜环菌二级菌种（原种）培养........................................3

4.2.2.1萌发菌二级菌种培养....................................................3

4.2.2.2蜜环菌二级菌种培养....................................................3

4.2.3萌发菌、蜜环菌三级菌种（栽培种）培养......................................3

4.2.3.1萌发菌三级菌种培养....................................................3

4.2.3.2蜜环菌三级菌种培养....................................................3

4.2.4菌种保藏..................................................................3

4.2.4.1一级菌种保藏..........................................................3

4.2.4.2二级菌种和三级菌种保藏................................................4

4.3蜜环菌枝培养..................................................................4

4.3.1树种选择..................................................................4

4.3.2培养时间..................................................................4

4.3.3培养方法..................................................................4

4.4蜜环菌材培养..................................................................4

4.4.1菌种准备..................................................................4

4.4.2树种选择..................................................................4

4.4.3培养时间..................................................................4

4.4.4木材准备..................................................................4

4.4.5场地选择..................................................................4

4.4.6培养方法..................................................................4

4.4.7培养管理..................................................................5

4.4.7.1调节湿度............................................................