T/WSWXH 002-2023 新疆主要果树和防护林腐烂病菌检测技术规程

ICS07.100

CCSB15/19

WSWXH

新疆维吾尔自治区微生物学会团体标准

T/WSWXH002—2023

代替T/WSWXH002-2022

新疆主要果树和防护林腐烂病菌检测

技术规程

RegulationforrapiddetectionofValsacankerofthefruittreeandtheshelterforest

ofXinjiang

2023-10-31发布2023-10-31实施

新疆维吾尔自治区微生物学会  发布

T/WSWXH002—2023

目次

前言..................................................................................II

1范围................................................................................1

2规范性引用文件......................................................................1

3术语和定义..........................................................................1

3.1PCR检测技术PolymeraseChainReaction...........................................1

3.2rDNA-ITS特异序列rDNAITSsequences.............................................1

3.3序列特异性引物Sequencespecificprimer..........................................1

3.4分生孢子器Pycnidium............................................................1

3.5分生孢子Conidium(复数conidia).................................................1

4方法原理............................................................................1

4.1分类地位........................................................................2

4.2鉴定依据........................................................................2

5主要设备及实验用具..................................................................2

5.1设备............................................................................2

5.2实验用具........................................................................2

6试验材料............................................................................2

6.1培养基..........................................................................2

6.2试剂............................................................................2

7现场检验............................................................................2

7.1取样方法........................................................................2

7.2病害症状........................................................................2

8实验室检测..........................................................................2

8.1分生孢子的显微镜检观察..........................................................2

8.2保湿培养........................................................................3

8.3病原菌的分离和纯化..............................................................3

8.4病原菌形态鉴定..................................................................3

8.5致病性测定......................................................................3

8.6分子生物学检测及鉴定............................................................3

9结果判定............................................................................3

附录A（资料性）培养基................................................................4

附录B（资料性）果树腐烂病形态特征....................................................5

附录C（资料性）苹果树腐烂病致病性测定方法及步骤......................................6

附录D（资料性）属专化型PCR引物检测方法..............................................7

附录E（资料性）种特异性PCR引物检测方法..............................................8

I