T/CACM 1481-2023 药用植物细胞培养技术规程

ICS11.120.99

CCSB38

团体标准

T/CACM1481—2023

药用植物细胞培养技术规程

Technicalregulationofmedicinalplantcellculture

2023-04-04发布2023-04-04实施

中华中医药学会发布

T/CACM1481—2023

目次

前言.................................................................................II

引言................................................................................III

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4工作区条件.........................................................................2

4.1概述...........................................................................2

4.2洗涤间.........................................................................2

4.3消毒间.........................................................................2

4.4培养基制备间...................................................................2

4.5接种间.........................................................................2

4.6培养间.........................................................................2

4.7细胞保存间.....................................................................2

5清洗与灭菌.........................................................................3

5.1清洗...........................................................................3

5.2灭菌...........................................................................3

6试剂与培养基.......................................................................3

6.1水.............................................................................3

6.2消毒灭菌剂.....................................................................4

6.3培养基.........................................................................4

6.4培养基的配制...................................................................4

7培养与保存.........................................................................5

7.1外植体的选择与处理.............................................................5

7.2接种...........................................................................5

7.3药用植物细胞的初代培养.........................................................6

7.4药用植物细胞的继代培养.........................................................6

7.5药用植物细胞的保存.............................................................6

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