DB43/T 1966-2020 大型溞繁殖毒性试验技术规程

ICS65.020

CCSB16

DB43

湖南省地方标准

DB43/T1966—2020

大型溞繁殖毒性试验技术规程

GuidelineforReproductionTestofDaphniamagnaStraus

2020-12-29发布2021-03-29实施

湖南省市场监督管理局发布

DB43/T1966—2020

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4环境条件···············································································································1

5仪器设备和器材······································································································2

6培养基··················································································································2

7试验溶液···············································································································2

8受试生物···············································································································2

9试验方法···············································································································3

10质量保证··············································································································4

附录A（资料性）ElendtM4培养液和M7培养基的制备····················································5

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DB43/T1966—2020

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DB43/T1966—2020

前言

本文件按照GB/T1.1—2020给出的规则起草。

本文件某些内容可能涉及专利。本文件发布机构不承担识别这些专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业标准化技术委员会归口。

本文件起草单位：湖南省植物保护研究所,长沙艾格里生物科技有限公司。

本文件主要起草人：陈昂、刘勇、张德咏、张战泓、罗杰、蒋桂芳、陈武瑛、熊浩、罗香文。

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DB43/T1966—2020

IV

DB43/T1966—2020

大型溞繁殖毒性试验技术规程

1范围

本文件规定了大型溞繁殖试验的环境条件、仪器和设备、培养基、试验方法、质量保证与质量控制、

数据处理等。

本文件适用于湖南省实验室内大型溞繁殖毒性试验。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

GB14942食品容器、包装材料用聚碳酸酯成型卫生标准

GB/T31270化学农药环境安全评价试验准则

NY/T1667农药登记管理术语

3术语和定义

下列术语和定义适用于本文件。

3.1

亲溞ParentAnimals

试验开始时用于繁殖的雌溞。

3.2

幼溞Offspring

试验期间亲溞所产的溞。

3.3

死亡Mortality

溞体不能游动，或轻晃试验容器，15s内溞的附肢或后腹部也未见活动。

4环境条件

4.1光照

每天光照16h，光照强度不超过1500lx。

4.2温度

试验的温度在18～22℃范围内。对于同一个试验，温度变化不得超过2℃。

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DB43/T1966—2020

4.3溶解氧

试验开始与试验期间，溶解氧浓度应大于3mg/L。

4.4pH

pH在6～9的范围内。正常情况下，任何一个试验中pH值的变化不应大于