GB/T 26350-2026 蝇类抗药性检测方法 家蝇生物测定法

ICS11.020

CCSC05

中华人民共和国国家标准

GB/T26350—2026

代替GB/T26350—2010

蝇类抗药性检测方法

家蝇生物测定法

Testmethodsofflyresistancetoinsecticides—

ThebioassaymethodsforMuscadomestica

2026⁃07⁃02发布2027⁃02⁃01实施

国家市场监督管理总局

国家标准化管理委员会发布

GB/T26350—2026

目次

前言··························································································································Ⅲ

1范围·······················································································································1

2规范性引用文件········································································································1

3术语和定义··············································································································1

4试虫·······················································································································2

4.1成虫·················································································································2

4.2幼虫·················································································································2

5试剂·······················································································································2

6仪器设备·················································································································2

7测试条件·················································································································2

8敏感基线法··············································································································2

8.1原理·················································································································2

8.2操作步骤···········································································································3

8.3死亡判断···········································································································4

8.4数据处理···········································································································4

8.5结果表述···········································································································5

8.6结果判定···········································································································5

9诊断剂量法··············································································································5

9.1原理·················································································································5

9.2操作步骤···········································································································5

9.3死亡判断···········································································································6

9.4结果表述···········································································································6

9.5结果判定···········································································································6

附录A（资料性）常用杀虫剂对敏感品系家蝇的致死剂量（浓度）············································7

Ⅰ

GB/T26350—2026

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

本文件代替GB/T26350—2010《蝇类抗药性检测方法家蝇生物测定法》，与GB/T26350—

2010相比，除结构调整和编辑性改动外，主要技术变化如下：

a）增加了术语“敏感品系”“待测种群”“人工饲料混药法”及其定义（见3.1、3.2、3.6），删除了术语

“F1代”（见2010年版的2.2），更改了术语“诊断剂量”（见3.3，2010年版的2.1）；

b）更改了试虫、试剂和仪器设备的要求（见第4章~第6章，2010年版的第3章~第5章）；

c）更改了测试条件（见第7章，2010年版的第6章）；

d）更改了敏感基线法中药膜法、点滴法的操作步骤（见8.2.1、8.2.2，2010年版的7.2）；

e）更改了诊断剂量法中药膜法、点滴法的操作步骤（见9.2.1、9.2.2，2010年版的8.2）；

f）增加了敏感基线法中人工饲料混药法的操作步骤（见8.2.3）；

g）增加了幼虫的死亡结果判定（见8.3.2）；

h）增加了数据处理的表述（见8.4）；

i）增加了诊断剂量法中人工饲料混药法的操作步骤（见9.2.3）。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由中华人民共和国国家卫生健康委员会提出并归口。

本文件起草单位：北京市疾病预防控制中心、中国农业大学、扬州大学、中国疾病预防控制中心传

染病预防控制所。

本文件主要起草人：李静、高希武、钱坤、周小洁、孟凤霞、佟颖、曾晓芃、尤春梅、宋敦伦。

本文件及其所代替文件的历次版本发布情况为：

——2011年首次发布为GB/T26350—2010；

——本次为第一次修订。

Ⅲ

GB/T26350—2026

蝇类抗药性检测方法

家蝇生物测定法

1范围

本文件规定了家蝇（Muscadomestica）对有机磷类、氨基甲酸酯类、拟除虫菊酯类、新烟碱类等杀虫剂

抗药性检测时，试虫、仪器设备和测试条件的要求，描述了敏感基线法和诊断剂量法的生物测定方法。

本文件适用于家蝇对杀虫剂抗药性的实验室生物测定。

2规范性引用文件

本文件没有规范性引用文件。

3术语和定义

下列术语和定义适用于本文件。

3.1

敏感品系susceptiblestrain

由于未曾接触过杀虫剂而保持对杀虫剂具有敏感状态，遗传上较均一的试虫种群。

3.2

待测种群testpopulation

从野外（现场）特定地点和时间采集的、需要进行杀虫剂敏感性测试的目标昆虫群体。

3.3

诊断剂量diagnosticdose

99%99.9%[99%LD99.9%

能杀死媒介生物敏感品系或个体的杀虫剂剂量致死量（99）或致死量

LD][99%LC99.9%LC]

（99.9）或浓度值致死浓度（99）或致死浓度（99.9）的数倍。

注：用以区分敏感和抗性种群。

3.4

点滴法topicalapplicationmethod

将定量药液点滴于供试昆虫体表的特定部位，通过杀虫剂穿透体壁进入体内引起昆虫中毒，以测

定药剂触杀毒力的生物测定方法。

3.5

药膜法residualfilmsmethod

将定量药液施布于载体的物体表面，并形成一层均匀的药膜，使试虫爬行接触药膜中毒致死，以测

定杀虫剂触杀毒力大小的生物测定方法。

3.6

人工饲料混药法dietincorporationmethod

用混有药剂的人工饲料饲养靶标昆虫的生物测定方法。

1