DB45/T 2312-2021 水牛体细胞核移植技术操作规程

ICS65.020.30

CCSB443

45

广西壮壮族族自自治区地方标准标准

DBB45/T2312—2021

水牛体细细胞核核移植植技术术操作作规程程

ThetTheeechnicalprotocothforleesomatincellcuutclearransferbuffinalo

2021--04-25发布2021-005-31实施

广西壮壮族自治区区市场监督督管理局发布布

DB45/T2312DB45/T—2021

目次

前言.................................................................................II.................................................................................前言

1范围...............................................................................1...............................................................................范围1

2规范性引用文件.....................................................................1.....................................................................规范性引用文件2

3术语和定义.........................................................................1.........................................................................术语和定义3

4缩略语.............................................................................1.............................................................................缩略语4

5药品试剂、耗材、设备和实验室.......................................................2.......................................................药品试剂、耗材、设备和实验室5

5.1药品试剂.......................................................................2.......................................................................药品试剂5.1

5.2耗材...........................................................................2...........................................................................耗材5.2

5.3设备...........................................................................2...........................................................................设备5.3

5.4实验室.........................................................................2.........................................................................实验室5.4

6卵母细胞和供体细胞准备.............................................................2.............................................................卵母细胞和供体细胞准备6

6.1工作液配制.....................................................................2.....................................................................工作液配制6.1

6.2卵母细胞的收集.................................................................2.................................................................卵母细胞的收集6.2

6.4卵母细胞体外成熟培养...........................................................3...........................................................卵母细胞体外成熟培养6.4

6.5卵母细胞成熟的判定.............................................................3.............................................................卵母细胞成熟的判定6.5

6.6供体细胞的培养.................................................................3.................................................................供体细胞的培养6.6

7胚胎生产...........................................................................3...........................................................................胚胎生产7

8胚胎分级...........................................................................4...........................................................................胚胎分级8

8.1A级...........................................................................4...........................................................................级A8.1

8.2B级...........................................................................4...........................................................................级B8.2

8.3C级.............................................