DB64/T 1638-2019 滩羊纯度PCR-mtDNA鉴定技术操作规程

DB64/T 1638-2019 DNA purification, primer design, and PCR reaction conditions are necessary to achieve reliable PCR-mtDNA results. In the case of Saanen goats, for instance, an analysis of 5 genes can provide valuable information on their origin, parentage, or individual health status. To avoid technical errors in obtaining the sample, DNA should be isolated using QIAamp DNA Mini Kit, followed by optimization of PCR condition and specific primers designed to avoid co-amplification of other goat breeds. The PCR product should be visualized using gel electrophoresis and the presence of a specifi