DB43/T 1950-2020 十字花科蔬菜鳞翅目害虫抗药性监测技术规程

ICS65.020

CCSB16

DB43

湖南省地方标准

DB43/T1095—2020

十字花科蔬菜鳞翅目害虫抗药性监测

技术规程

Technicalregulationofinsecticideresistancemonitoringof

lepidopterousinsectpestsoncruciferousvegetables

2020-12-29发布2021-03-29实施

湖南省市场监督管理局发布

DB43/T1950—2020

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4试剂与材料············································································································2

5仪器设备···············································································································2

6试验步骤···············································································································3

7数据统计与分析······································································································4

8抗性水平评估·········································································································4

9监测档案···············································································································5

附录A（资料性）斜纹夜蛾对部分杀虫剂敏感毒力基线（点滴法）······································6

附录B（资料性）甜菜夜蛾对部分杀虫剂敏感毒力基线（夹毒叶片法）································7

附录C（资料性）小菜蛾对部分杀虫剂敏感毒力基线（浸叶法）·········································8

附录D（资料性）十字花科蔬菜鳞翅目害虫抗药性监测档案···············································9

I

DB43/T1950—2020

II

DB43/T1950—2020

前言

本文件按照GB/T1.1—2020给出的规则起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别这些专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业标准化技术委员会归口。

本文件起草单位：湖南省植物保护研究所、长沙县农业农村局、湖南农业大学、郴州市农业科学研

究所、邵阳市植保植检站、桃江县植保植检站。

本文件主要起草人：符伟、刘箐、彭可为、唐涛、王培、周玲红、彭智伟、马明勇、丁朝辉、魏林、

张卓。

III

DB43/T1950—2020

IV

DB43/T1950—2020

十字花科蔬菜鳞翅目害虫抗药性监测技术规程

1范围

本文件规定了小菜蛾（PlutellaxylostellaLinnaeus）、甜菜夜蛾（SpodopteraexiguaHiibner）、斜纹夜

蛾（SpodopteralituraFabricius）抗药性监测的术语和定义、试剂与材料、仪器设备、试验步骤、数据

统计与分析、抗性水平评估及监测档案。

本文件适用于小菜蛾、甜菜夜蛾、斜纹夜蛾对常用杀虫剂的抗药性监测。其它十字花科蔬菜鳞翅目

害虫抗药性监测参照本标准执行。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

NY/T1154.1农药室内生物测定试验准则第1部分：点滴法

NY/T1154.2农药室内生物测定试验准则第2部分：夹毒叶片法

NY/T1154.14农药室内生物测定试验准则第14部分：浸叶法

3术语和定义

下列术语和定义适用于本文件。

3.1

抗药性insecticideresistance

原来易感某种杀虫剂的昆虫种群，不再能被该剂常用量杀死的特性。

3.2

敏感基线susceptibilitybaseline

通过生物测定方法得到的害虫敏感品系（或种群）对杀虫剂的剂量反应曲线。

3.3

致死中浓度（LC50）medianlethalconcentration

药剂杀死生物群体中一半个体所需要的浓度。

3.4

点滴法topicalapplicationmethod

将定量的药剂滴在昆虫体壁的一定部位，使药剂透过表皮进入体内，以测定药剂触杀效果的生物测

定方法。

3.5

夹毒叶片法leaf-sandwichmethod

将定量的药剂夹入叶面，使药剂通过昆虫取食进入体内，以测定药剂喂毒效果的生物测定方法。

1

DB43/T1950—2020

3.6

浸叶法leaf-dippingmethod

将浸过药液的叶碟置于含有琼脂或保湿滤纸上，接入靶标昆虫，以测定药剂触杀和喂毒效果的生物

测定方法。

4试剂与材料

4.1试剂

试验试剂主要为：

——丙酮

——乙醇

——TritonX-100

——蒸馏水

4.2生物试材

4.2.1供试昆虫

供试昆虫为：

——小菜蛾，田间采集，经室内饲养的F1代大小一致的3龄幼虫；

——甜菜夜蛾，田间采集，经室内饲养的F1代大小一致的3龄幼虫；

——斜纹夜蛾，田间采集，经室内饲