DB32/T 3979-2021 实验用 斑马鱼 饲育技术条件

ICS07.080

CCSA40

32

江苏省地方标准

DB32/T3979—2021

实验用斑马鱼饲育技术条件

Technicalrequirementsforbreedinglaboratoryzebrafish

2021-02-03发布2021-03-03实施

江苏省市场监督管理局发布

DB32/T3979-2021

目次

前言................................................................................III

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4饲育鱼房工艺布局...................................................................1

4.1饲育鱼房总体要求...............................................................1

4.2饲育鱼房功能分区...............................................................2

5饲育设施...........................................................................2

5.1流水饲育系统...................................................................2

5.2静水饲育系统...................................................................3

6饲育环境条件.......................................................................3

6.1温度...........................................................................3

6.2湿度...........................................................................3

6.3空气...........................................................................3

6.4环境噪音.......................................................................3

6.5光照...........................................................................3

6.6饲育用水.......................................................................4

6.7流水循环速度...................................................................4

6.8日换水量.......................................................................4

7饲育密度与饵料及饲喂...............................................................4

7.1饲育密度.......................................................................5

7.2饲育饵料.......................................................................5

7.3饲喂...........................................................................5

8繁育和遗传资源保存.................................................................5

8.1繁育安排.......................................................................5

8.2新创制遗传资源保存.............................................................5

9伦理维护...........................................................................6

9.1福利保障.......................................................................6

9.2饲育人员技能...................................................................6

9.3生长记录.......................................................................6

9.4标识...........................................................................6

9.5安乐死.........................................................................6

10质量规范..........................................................................6

10.1实验用斑马鱼来源..............................................................6

10.2胚胎的质量....................................................................6

10.3仔鱼的质量....................................................................6

10.4幼鱼与成鱼的质量..............................................................6

I

DB32/T3979—2021

附录A（资料性）草履虫培育...........................................................8

附录B（资料性）丰年虫幼虫孵化及脱盐处理...........................................