DB4401/T 85-2020 红掌组培种苗生产技术规程

ICS65.020.20

B05

DB4401

广州市地方标准

DB4401/T85—2020

代替DB440100/T169—2014

红掌组培种苗生产技术规程

TheproductionregulationofAnthuriumandreanuminplantletvitro

2020-06-08发布2020-07-01实施

广州市市场监督管理局发布

DB4401/T85—2020

目    次

前言.....................................................................................................................................................................Ⅲ

1范围.................................................................................................................................................................1

2规范性引用文件.............................................................................................................................................1

3术语和定义.....................................................................................................................................................1

4设备设施及化学药剂.....................................................................................................................................1

4.1设备设施.................................................................................................................................................1

4.2化学药剂.................................................................................................................................................2

5消毒灭菌.........................................................................................................................................................3

5.1洗涤室消毒灭菌.....................................................................................................................................3

5.2接种室消毒灭菌.....................................................................................................................................3

5.3培养室消毒灭菌.....................................................................................................................................3

5.4超净工作台消毒灭菌.............................................................................................................................3

5.5培养基的消毒灭菌.................................................................................................................................3

5.6接种器具消毒灭菌.................................................................................................................................3

5.7污染瓶苗消毒灭菌.................................................................................................................................3

6培养室环境条件控制.....................................................................................................................................4

6.1温度.........................................................................................................................................................4

6.2湿度.........................................................................................................................................................4

6.3光照.........................................................................................................................................................4

7组培快繁技术.................................................................................................................................................4

7.1培养基配制.............................................................................................................................................4

7.2外植体选择.............................................................................................................................................4

7.3外植体消毒灭菌.....................................................................................................................................4

7.4接种.........................................................................................................................................................5

7.5愈伤组织诱导培养.................................................................................................................................5

7.6愈伤组织增殖培养.................................................................................................................................5

7.7芽分化培养.............................................................................................................................................5

7.8不定芽的继代增殖培养.........................................................................................................................5

7.9生根培养.................................................................................................................................................5

7.10组培苗炼苗...........................................................................................................................................6

8出瓶移栽技术.................................................................................................................................................6

8.1组培苗清洗消毒.....................................................................................................................................6

8.2移植基质.................................................................................................................................................6

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DB4401/T85—2020

8.3移植容器选择.........................................................................................................................................6

8.4种植.........................................................................................................................................................6

8.5日常管理.................................................................................................................................................7

8.6病虫害防治.............................................................................................................................................9

9包装.................................................................................................................................................................9

9.1包装材料...............................................................................................................................................10

9.2包装方法...............................................................................................................................................10

附录A（资料性附录）红掌组培快繁培养基基本配方.............................................................................11

附录B（资料性附录）红掌组培快繁常用植物生长调节物质.................................................................12

II