DB43/T 2332-2022 茶白星病原菌检测技术规程

ICS67.140.10

CCSB35

43

湖南省地方标准

DB43/T2332—2022

茶白星病原菌检测技术规程

TechnicalregulationfordetectionofElsinoeleucospila

2022-05-06发布2022-08-06实施

湖南省市场监督管理局发布

DB43/T2332—2022

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4样品采集···············································································································1

5形态检测···············································································································2

6分子检测···············································································································2

附录A（资料性）茶白星病的田间症状··········································································4

附录B（资料性）茶白星病菌的菌落、菌丝与孢子形态·····················································5

附录C（资料性）茶白星病菌基因序列片段····································································6

参考文献····················································································································7

I

DB43/T2332—2022

II

DB43/T2332—2022

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业农村标准化技术委员会归口。

本文件起草单位：湖南省茶叶研究所。

本文件主要起草人：周凌云、杨辉、杨文波、向芬、刘红艳、李维、银霞、曾泽萱。

III

DB43/T2332—2022

IV

DB43/T2332—2022

茶白星病原菌检测技术规程

1范围

本文件规定了茶白星病原菌的术语和定义、样品采集、形态检测和分子检测。

本文件适用于茶白星病菌-茶痂囊腔菌（Elsinoeleucospila）的检测。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

GB/T6682分析实验室用水规格和试验方法

GB/T8302茶取样

SB/T10314—1999采样方法及检验规则

NY/T3862茶云纹叶枯病监测技术规程

3术语和定义

本文件没有需要界定的术语和定义。

4样品采集

4.1采样特征

4.1.1发生部位

茶白星病症状可见于0～2000m海拔茶区的茶树嫩梢、成叶、老叶与花苞等茶树组织。

4.1.2初期症状

初期病斑为针尖大小的红褐色小点，随即围绕该点出现多个红褐色斑点呈卫星状分布，斑点直径生

长至2.5mm大小，红褐色圆形病斑边缘具有暗褐色至紫褐色隆起线，中间凹陷、平伏或凸起。

4.1.3后期症状

发病后期病斑由红褐色渐变为黑褐色，边缘凸起，中间白色，呈疮痂状，病斑呈数个至上百个连片

分布，严重时病斑相互融合成不规则的大病斑。大病斑常呈黑色凸起，中间呈灰白色（见附录A）。

4.2采样