DB43/T 2224-2021 规模猪场非洲猪瘟监测样品采集及检 测技术规程

ICS65.020

CCSB16

43

湖南省地方标准

DB43/T2224—2021

规模猪场非洲猪瘟监测样品采集

及检测技术规程

Technicalspecificationformonitoringsamplecollectionanddetection

ofAfricanswinefeverinlargescalepigfarms

2021-11-09发布2022-01-09实施

湖南省市场监督管理局发布

DB43/T2224—2021

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4要求·····················································································································1

5样品采集···············································································································2

6样品包装与运输······································································································3

7检测·····················································································································3

8阳性结果处置·········································································································3

9废弃物处理············································································································4

10档案记录··············································································································4

附录A（资料性）非洲猪瘟环境样品采样位置与数量表·····················································5

附录B（资料性）非洲猪瘟监测样品采样记录表······························································6

I

DB43/T2224—2021

II

DB43/T2224—2021

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业标准化技术委员会归口。

本文件起草单位：湖南省畜牧兽医研究所、湖南鑫广安农牧股份有限公司、怀化市畜牧水产事务中

心、安仁县旺峰养殖专业合作社。

本文件主要起草人：杨俊、王慧、刘伯承、杜丽飞、宋武、周望平、杨爱梅、洪蕾、江新明、刘俊

琦、蒋战波、冯小花、陈一峰、彭苗苗、聂后福、袁旭鹏。

III

DB43/T2224—2021

IV

DB43/T2224—2021

规模猪场非洲猪瘟监测样品采集及检测技术规程

1范围

本文件规定了规模猪场非洲猪瘟监测样品采集及检测技术的术语和定义、要求、样品采集、样品包

装与运输、检测、阳性结果处置、废弃物处理和档案记录等要求。

本文件适用于湖南省规模猪场非洲猪瘟监测样品的采集和检测。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

GB∕T18648非洲猪瘟诊断技术

GB19489实验室生物安全通用要求

NY/T541兽医诊断样品采集、保存与运输技术规范

NY/T1948兽医实验室生物安全要求通则

DB43/T1670非洲猪瘟病毒检测免核酸提取荧光PCR法

高致病性动物病原微生物菌（毒）种或者样本运输包装规范（农业部公告第503号）

3术语和定义

下列术语和定义适用于本文件。

3.1

监测样品（monitoringsample）

取自生猪或环境，拟通过检测