DB37/T 3573-2019 牛支原体诊断技术规程

ICS11.220

B41

DB37

山东省地方标准

DB37/T3573—2019

牛支原体诊断技术规程

CodeofPracticefortheDiagnosisofMycoplasmaBovis

2019-05-29发布2019-06-29实施

山东省市场监督管理局发布

DB37/T3573—2019

目次

前言................................................................................II

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语与定义.........................................................................1

4试剂及仪器.........................................................................1

4.1试剂...........................................................................1

4.2仪器...........................................................................2

5样品采集与处理.....................................................................2

5.1疑似牛支原体感牛只的判断.......................................................2

5.2疑似病例样品的采集.............................................................2

5.3样品处理.......................................................................2

6微生物学检测法.....................................................................2

6.1液体培养.......................................................................2

6.2菌落形态观察...................................................................2

6.3Dienes染色.....................................................................3

6.4生化试验.......................................................................3

6.5结果判定.......................................................................3

7核酸检测法.........................................................................3

7.1DNA模板的制备..................................................................3

7.2PCR检测........................................................................3

7.3凝胶电泳.......................................................................4

7.4质量控制.......................................................................4

7.5结果判定.......................................................................4

8废弃物的处理和检测过程中安全措施...................................................4

9操作注意事项.......................................................................5

附录A（规范性附录）培养基的制备方法............................................6

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