DB37/T 3630-2019 小麦普通腥黑穗病菌分子检测方法

ICS65.020.01

B16

DB37

山东省地方标准

DB37/T3630—2019

小麦普通腥黑穗病菌分子检测方法

MoleculardetectionmethodsofTilletiafoetida&Tilletiacaries

2019-07-23发布2019-08-23实施

山东省市场监督管理局发布

DB37/T3630—2019

目次

前言................................................................................II

1范围...............................................................................1

2术语和定义.........................................................................1

3仪器设备...........................................................................1

4主要试剂...........................................................................1

5分子检测...........................................................................1

5.1小麦种子中小麦普通腥黑穗病菌冬孢子基因组DNA提取...............................1

5.2菌瘿中冬孢子基因组DNA提取.....................................................2

5.3引物序列.......................................................................2

5.4PCR扩增........................................................................2

5.5电泳检测.......................................................................2

5.6检测结果判断...................................................................3

6结果判定...........................................................................3

7样品保存...