DB37/T 3571-2019 牛副结核病γ干扰素ELISA诊断技术

ICS11.220

B41

DB37

山东省地方标准

DB37/T3571—2019

牛副结核病γ干扰素ELISA诊断技术

Diagnosisonbovineparatuberculosisbyenzyme-linkedimmunoassayofγ-interferon

2019-05-29发布2019-06-29实施

山东省市场监督管理局发布

DB37/T3571—2019

目次

前言................................................................................II

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4试验原理...........................................................................1

5试剂及仪器.........................................................................1

5.1材料...........................................................................1

5.2仪器...........................................................................2

6检测方法...........................................................................2

6.1试剂配制.......................................................................2

6.2采血...........................................................................2

6.3全血培养.......................................................................2

6.4检测步骤.......................................................................2

7结果判定...........................................................................2

7.1样品结果计算...................................................................2

7.2阴阳性结果判定标准.............................................................3

8废弃物的处理.......................................................................3

9检测过程中防止交叉污染的措施.......................................................3

10操作注意事项......................................................................3

附录A（资料性附录）血液处理所需材料............................................4

附录B（资料性附录）ELISA试验所需试剂...........................................5

附录C（资料性附录）检测所需仪器................................................6

附录D（规范性附录）检测所用试剂的配制..........................................7

I