DB35/T 1280-2012 大豆疫霉菌分子检测技术规程

ICS65.020

B16

DB35

福建省地方标准

DB35/T1280—2012

大豆疫霉菌分子检测技术规程

StandardprotocolformoleculardetectionofPhytophthorasojae

2012-11-02发布2013-02-01实施

福建省质量技术监督局发布

福建省地方标准

大豆疫霉菌分子检测技术规程

DB35/T1280—2012

*

2013年1月第一版2013年1月第一次印刷

DB35/T1280—2012

目次

前言...............................................................................III

1范围..............................................................................1

2规范性引用文件....................................................................1

3术语和定义........................................................................1

4原理..............................................................................1

5材料与试剂........................................................................1

5.1主要仪器与器材................................................................1

5.1.1PCR检测仪.................................................................1

5.1.2凝胶成像仪................................................................2

5.1.3台式离心机................................................................2

5.1.4涡旋混匀器................................................................2

5.1.5电泳仪....................................................................2

5.2主要试剂......................................................................2

5.2.1苯酚；氯仿；异戊醇；乙醇..................................................2

5.2.2二甲基亚砜................................................................2

5.2.3氢氧化钠..................................................................2

5.2.4脱脂奶粉..................................................................2

5.2.5PCR常规试剂...............................................................2

6采样..............................................................................2

6.1采样工具......................................................................2

6.2样品采集......................................................................2

6.2.1大豆植株..................................................................2

6.2.2土壤......................................................................2

6.3样品贮运......................................................................2

6.4样品存放......................................