DB43/T 1959-2020 辣椒炭疽病菌检测技术规程

ICS65.020

CCSB16

DB43

湖南省地方标准

DB43/T1599—2020

辣椒炭疽病菌检测技术规程

TechnicalRegulationsforDetectionofColletotrichumcomplex

ofCapsciumannuumL.

2020-12-29发布2021-03-29实施

湖南省市场监督管理局发布

DB43/T1959—2020

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4原理·····················································································································1

5仪器设备与试剂······································································································2

5.1仪器设备·········································································································2

5.2主要试剂·········································································································2

6采样·····················································································································2

6.1样品采集·········································································································2

6.2样品贮运与存放································································································2

7操作方法···············································································································2

7.1样品DNA制备···································································································3

7.2检测···············································································································3

8结果判定···············································································································4

9样品保存与处理······································································································4

附录A（资料性）辣椒炭疽病菌相关资料·······································································5

附录B（规范性）辣椒炭疽病菌样本鉴定报告·································································7

I

DB43/T1959—2020

II

DB43/T1959—2020

前言

本文件按照GB/T1.1—2020给出的规则起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别这些专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业标准化技术委员会归口。

本文件起草单位：湖南省植物保护研究所。

本文件主要起草人：谭新球、张鑫、满益龙、陈岳、郑立敏、张卓、欧阳超、刘思珍。

III

DB43/T1959—2020

IV

DB43/T1959—2020

辣椒炭疽病菌检测技术规程

1范围

本文件规定了辣椒炭疽病菌高效、快速检测和种类鉴定的样品制备、检测技术和操作程序。