DB2302/T 012-2021 马铃薯试管苗继代培养技术规程

ICS650.020.40

B21

DB2302

黑龙江省齐齐哈尔市地方标准

DB2302/T012—2021

马铃薯试管苗继代培养技术规程

2021-12-26发布2022-01-26实施

齐齐哈尔市市场监督管理局发布

DB2302/T012—2021

目次

前言...............................................................................III

引言................................................................................IV

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

3.1试管苗.........................................................................1

3.2脱毒试管苗.....................................................................1

3.3继代...........................................................................1

3.4继代培养.......................................................................1

4材料选择...........................................................................1

4.1症状学观察.....................................................................2

4.1.1繁殖材料...................................................................2

4.1.2症状学观察.................................................................2

4.2类病毒与病毒病检测.............................................................2

4.2.1RT-PCR法检测...............................................................2

4.2.2DAS-ELISA法检测............................................................3

4.3材料选择.......................................................................3

5培养基制备.........................................................................3

5.1器材与试剂.....................................................................3

5.2操作程序.......................................................................3

5.2.1洗涤.......................................................................3

5.2.2MS培养基药品母液配制和保存.................................................3

5.2.3MS培养基制备...............................................................3

5.2.4培养基的分装和灭菌.........................................................4

6茎段剪切...........................................................................4

6.1器材与试剂.....................................................................4

6.2操作程序.......................................................................4

6.2.1无菌操作室内消毒杀菌.......................................................4

6.2.1.1操作前消毒杀菌.........................................................4

6.2.1.2操作中消毒.............................................................4

6.2.2茎段剪切...................................................................4

7组织培养...........................................................................5

7.1器材...........................................................................5

7.2组培条件.......................................................................5

I

DB2302/T012—2021

8档案管理...........................................................................5

附录A（规范性附录）MS培养基配方....................................................6

II