DB32/T 3762.13-2021 新型冠状病毒检测技术规范 第13部分：叠氮溴化丙锭-荧光PCR检测程序

ICS11.020

C62

DB32

江苏省地方标准

DB32/T3762.13—2021

新型冠状病毒检测技术规范

第13部分：叠氮溴化丙锭-荧光PCR

检测程序

TechnicalspecificationsforSARS-CoV-2detection

Part13:PMA-qRT-PCRtestprocedure

2021-12-09发布2022-01-09实施

江苏省市场监督管理局发布

DB32/T3762.13—2021

目次

前言............................................................................II

1范围..............................................................................1

2规范性引用文件....................................................................1

3术语和定义........................................................................1

4环境与设施........................................................................1

5设备与耗材........................................................................1

6试剂与材料........................................................................1

7试验技术要点......................................................................2

8样本前处理........................................................................2

9样本PMA预处理与核酸提取.........................................................2

10扩增体系配制与扩增.................................................................2

11阈值设定...........................................................................3

12样本前处理...