GB/T 4789.32-2002 食品卫生微生物学检验 大肠菌群的快速检测

ICS07.100.30

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中华人民共和国国家标准

GB/T4789.32-2002

食品卫生微生物学检验

大肠菌群的快速检测

Microbiologicalexaminationforfoodhygiene

rapiddetectionofcoliformbacteria

2002一06一13发布2002一10一01实施

中华人民共和国发布

国家质量监督检验检疫总局

GB/T4789.32-2002

目次

前言···········································”···················“.····································.，······……I

引言················································································································……II

1范围············································································································……1

2规范性引用文件············································································。········”······……1

3术语和定义····，······.，················“·····”·“.·，··.·，·，’·····································”·····……1

4原理················································”··············，··························”··············……1

5试剂和培养基·······················································································.·，······……1

6设备和器材·························································……‘····································……2

7检验程序···························································，···········································”……3

8样品的制备···································································································……3

9大肠菌群的MPN计数····················································································……’二3

10大肠菌群的菌落计数······，··················”······························································……4

附录A(规范性附录)大肠菌群最可能数(MPN)检索表················································……5

Gs/T4789-32-2002

前言

本标准规定了食品中大肠菌群的快速检验方法。本标准规定使用的培养基MUGal肉汤中的4一甲

基伞形酮-3(-D一半乳糖昔与美国联邦法规40，第141节，21款(CodeofFederalRegulations40,Part

141.21)规定的检验大肠菌群所用的MI琼脂的主要成分(4一甲基伞形酮一件D一半乳糖昔)是同一物质。

本标准关于大肠菌群的菌落计数，参考了ISO4832c1991(EA微生物学—大肠菌群菌落计数法

通则》(Microbiology-Generalguidancefortheenumerationofcoliforms-colonycounttechnique),

本标准中的附录A是规范性附录。

本标准由国家质量监督检验检疫总局提出并归口。

本标准主要起草单位:镇江出人境检验检疫局、中国标准化协会、镇江质量技术监督局。

本标准主要起草人:甄宏太、李平、张秀春A继福。

Ga/T4789.32-2002

引言

大肠菌群系指一群能发醉乳糖、产酸或醛，并产生各半乳糖昔酶需氧或兼性厌氧的革兰氏阴性无芽

抱杆菌。该菌主要来源于人畜粪便，故以此作为粪便污染指标来评价食品的卫生质量，推断食品中有否

污染肠道致病菌的可能。如果大肠菌群存在于食品中，表明未作有效的消毒处理、加工后保存条件不良

或消毒后又受到污染。快速检验这些细菌，有助于食品的卫生管理，维护消费者的食用安全。

GB/T4789.32-2002

食品卫生微生物学检验

大肠菌群的快速检测

范围

本标准规定了食品中大肠菌群的快速检验和计数方法。

本标准适用于食品中大肠菌群的快速检验和计数。

规范性引用文件

下列文件中的条款通过本标准的引用而成为本标